Understanding the Cost of Long Chain Primers in Custom DNA Oligonucleotides

Custom DNA oligonucleotides are essential in molecular biology, enabling researchers to create primers for various applications, including PCR, gene cloning, and sequencing. However, the cost of long chain primers can be significantly higher than that of short chain primers. This article explores the reasons behind this pricing discrepancy and the purification methods used in the synthesis process offered by Tsingke.

Why Long Chain Primers Are More Expensive

One of the primary reasons long chain primers, especially those exceeding 90 bases, are more costly is the increased amount of reagents required for their synthesis. Longer primers demand more raw materials and involve complex synthesis processes that elevate production costs. The additional time and resources necessary to manufacture these longer chains contribute to the overall price, making them a more expensive option compared to their shorter counterparts.

Purification Methods for Custom DNA Oligonucleotides

Purification is a critical step in ensuring the quality and functionality of custom DNA oligonucleotides. Tsingke employs various methods to purify DNA oligos, each with its advantages:

DSL (Desalting): This method effectively removes salts and small impurities from the oligos, ensuring they are suitable for downstream applications.

OPC (Oligonucleotide Purification Cartridge): OPC provides a quick and efficient way to purify oligos by binding them to a cartridge and washing away contaminants.

PAGE (Polyacrylamide Gel Electrophoresis): PAGE separates oligos based on their size and charge, allowing for high-purity oligo isolation.

HPLC (High-Performance Liquid Chromatography): This technique is known for producing highly purified oligos, making it ideal for critical applications where purity is paramount.

Dual PAGE & HPLC: Combining both methods offers a comprehensive purification strategy, ensuring maximum purity and yield of the synthesized oligos.

Conclusion

In the world of custom DNA oligonucleotides, understanding the cost factors and purification methods is essential for researchers. Long chain primers, while more expensive, provide unique advantages in specific applications, justifying their price. With various purification methods available, Tsingke ensures the quality of their DNA oligonucleotides, enhancing the reliability of scientific research and experimentation.

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